Effectene transfection reagent manual

The following paramenters can be optimized to increase transfection efficiency when using Effectene Transfection Reagent. Optimize the Effectene Reagent to DNA ratio. If the ratio of Effectene Reagent to DNA is suboptimal, the overall charge of the complexes may be negative, neutral or strongly positive, which can lead to inefficient adsorption to the cell surface. Effectene Reagent is a unique non-liposomal lipid formulation. Effectene Reagent is used in conjunction with an Enhancer and a DNA-condensation buffer (Buffer EC) to achieve high transfection efficiencies. In the first step of Effectene–DNA complex formation, the DNA is condensed by interaction with the Enhancer in a defined buffer system. The ratio of Effectene Transfection Reagent to DNA required for optimal performance may vary, depending on the cell line and gene target. Table 2 provides suggestions for optimizing the ratio of DNA to Effectene Transfection Reagent. The ratio of DNA to Enhancer () provided in the protocol should not be changed.

SatisFection Transfection Reagent Instruction Manual Catalog #, #, and # Revision B Research Use Only. Not for Use in Diagnostic Procedures. LIMITED PRODUCT WARRANTY This warranty limits our liability to replacement of this product. No other warranties of any kind, express. 6 K4®Transfection System Manual EN Transfection of cells with DNA - standard protocol for well-format - 1. Plate - x 10 5 adherent cells (starting-point x 10 5) or x 10 5 suspension cells in a single well of a well dish in ml of suitable complete. TECHNICAL MANUAL ViaFect™ Transfection Reagent Instructions for Use of Products E, E and E Promega Corporation · Woods Hollow Road · Madison, WI USA · Toll Free in USA · · Fax 1 www.doorway.ru TM · Revised 7/

3. Briefly vortex the transfection reagent and add 2µL to the diluted DNA. Mix immediately by pipetting or vortexing. 4. Incubate minutes at room temperature. 5. Add µL of the transfection reagent/DNA mixture drop-wise to each well. Do not remove the growth medium from the cells before adding the transfection reagent/DNA mixture. 6. If so, vary DNA (μg): Lipofectamine® (μl) ratios from to If using a different transfection reagent, please consult the product manual. Dilution times or complexing incubation period was too short: For Lipofectamine® , we recommend that the DNA and transfection reagent be diluted separately and incubated for five minutes. In a study involving primary human myoblasts, the effect of transfection efficiencies was compared using different nucleic acid ratios to transfection reagents including FuGENE 6, Effectene, and ExGen (a PEI-based reagent) (Arnold et al., ). One remarkable finding from the study was that transfection efficiency might not necessarily.